The most conspicuous attribute of living organisms is that they are complicated and highly organized. The cells of which they are composed possess intricate internal structures containing many kinds of the complex molecule and chemical reactions. Biochemistry makes significant contributions to the fields of cell biology, physiology, immunology, microbiology, pharmacology, and toxicology, as well as the fields of inflammation, cell injury, and cancer. These close relationships emphasize that life, as we know it, depends on biochemical reactions and processes. The term ‘Biochemistry’ was first introduced by the German Chemist Carl Neuberg in 1903. Biochemistry is both a life science and a chemical science – it explores the chemistry of living organisms and the molecular basis for the changes occurring in living cells. Biochemistry has become the foundation for understanding all biological processes. In the ever-expanding knowledge of Biochemistry, it is very difficult on the part of a single individual to go through the various bigger volume of textbooks on the experimental part of biochemistry and its investigations. The purpose of writing this book is to bring out a practical manual that caters for the need of graduate and Postgraduate students and Research scholars. This book covers the basic and advanced techniques of biochemistry and Life Sciences which will be very useful for UG, PG and Research Scholars in Biochemistry, Microbiology, and Biotechnology. This book is written in a simple and concise way in keeping the view of students, research scholars and college professors to understand and apply various practical concepts in Biochemistry, Microbiology, Biotechnology and Life sciences.