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Practical manual of Biochemistry, Microbiology and Cell Biology – Dr. R. Rajakumar,

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The contents of this manual have been compiled after extensive consultation of standard textbooks, laboratory manuals, scientific journals, university syllabi and established laboratory protocols. Every effort has been made to ensure scientific accuracy, clarity and relevance.

Description

The manual comprehensively covers the practical exercises prescribed in the university syllabus. The Biochemistry section includes laboratory safety, preparation of reagents and buffers, qualitative analysis of carbohydrates, proteins and amino acids, estimation techniques, preparation of biological samples and isolation of important biomolecules. The Microbiology section focuses on microbiological laboratory practices, sterilization techniques, preparation of culture media, isolation and cultivation of microorganisms, microbial enumeration, staining methods and biochemical characterization of bacteria. The Cell Biology section introduces students to microscopy, preparation of temporary mounts, observation of plant and animal cells, mitotic cell division, subcellular fractionation, cell organelles and other essential cellular techniques that strengthen the understanding of cell structure and function.

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